产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ATG12 (A98) polyclonal antibody（KU）

货号: AP0759

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0759

Product Name :ATG12 (A98) polyclonal antibody（KU）

Swiss-Prot :O94817

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG12 (A98) polyclonal antibody detects endogenous levels of ATG12 protein.

BiowMW :~ 20, 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :Ubiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L

Immunogen :Synthetic peptide, corresponding to Human ATG12.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATG12 (A98) polyclonal antibody at 1:500 dilution Lane1:SP2/0 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:PC3 whole cell lysate(40ug) Lane4:SHSY5Y whole cell lysate(40ug) Lane5:HCT116 whole cell lysate(40ug)

Immunohistochemistry of paraffin-embedded Human Liver cancer using ATG12 antibody at dilution of 1:50.