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Smad2/3 (S2) polyclonal antibody（KU）

货号: AP0444

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0444

Product Name :Smad2/3 (S2) polyclonal antibody（KU）

Swiss-Prot :Q15796

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IF

Application_all :WB: 1:1000~1:2000 IF 1:50~1:200

Background :Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Smad2/3 (S2) pAb detects endogenous levels of Smad2/3 protein.

BiowMW :~ 52, 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling,

Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Immunogen :Synthetic peptide, corresponding to Human Smad2/3.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Smad2/3 (S2) pAb at 1:1000 dilution Lane1:PC12 whole cell lysate(30ug) Lane2:CT-26 whole cell lysate(30ug) Lane3:Hela whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug) Lane5:EC9706 whole cell lysate(30ug)

Immunofluorescence analysis of Hela cells using Smad2/3 (S2) pAb at dilution of 1:200.

Immunoprecipitation of HEK293T cell lysates using Smad2/3 (S2) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Smad2/3 (S2) pAb.

TGF-β induces M2-like macrophage polarization via SNAIL-mediated suppression of a pro-inflammatory phenotype

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID27418133
Silencing of HMGA2 suppresses cellular proliferation, migration, invasion, and epithelial–mesenchymal transition in bladder cancer

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IF0

SpeciesHuman

PMID26684800
Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice

JournalJournal Of Agricultural And Food Chemistry

ApplicationWB

IF3.571

SpeciesMouse

PMID29224337
Heat stress during late pregnancy of sows influences offspring longissimus dorsi muscle growth at weaning

JournalResearch In Veterinary Science

ApplicationWB

IF2.534

SpeciesPig(Sow)

PMID33765607