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Smad2/3 (S2) polyclonal antibody（KU）

货号: AP0444

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0444

Product Name :Smad2/3 (S2) polyclonal antibody（KU）

Swiss-Prot :Q15796

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IF

Application_all :WB: 1:1000~1:2000 IF 1:50~1:200

Background :Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Smad2/3 (S2) pAb detects endogenous levels of Smad2/3 protein.

BiowMW :~ 52, 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling,

Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Immunogen :Synthetic peptide, corresponding to Human Smad2/3.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Smad2/3 (S2) pAb at 1:1000 dilution Lane1:PC12 whole cell lysate(30ug) Lane2:CT-26 whole cell lysate(30ug) Lane3:Hela whole cell lysate(30ug) Lane4:HEK293T whole cell lysate(30ug) Lane5:EC9706 whole cell lysate(30ug)

Immunofluorescence analysis of Hela cells using Smad2/3 (S2) pAb at dilution of 1:200.

Immunoprecipitation of HEK293T cell lysates using Smad2/3 (S2) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Smad2/3 (S2) pAb.

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