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PKC δ (phospho-T505) polyclonal antibody（KU）

货号: AP0291

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :AP0291

Product Name :PKC δ (phospho-T505) polyclonal antibody（KU）

Swiss-Prot :Q05655

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :Protein Kinase C delta (PKC delta) is a 78 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC delta is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of multiple cellular processes including cell cycle progression and apoptosis. Autophosphorylation of serine 664 (serine 662 in mouse and rat) contributes to PKC delta activity. Serum dependent phosphorylation of serine 664 is mediated by mTOR pathway, is protected from dephosphorylation by phosphorylated threonine 505 in the activation loop, and is implicated in prolactin signaling.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 97% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PKC δ (T505) polyclonal antibody detects endogenous levels of PKC δ protein only when phosphorylated at Thr505.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Protein Kinase C Signaling,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; PKCδ

Immunogen :Synthetic phosphopeptide derived from human PKC δ around the phosphorylation site of Threonine 505.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of PKC δ (phospho-T505) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate Lane2:MCF-7 treated with LPS(100ng/ml) for 5 minutes whole cell lysate Lane3:MCF-7 treated with LPS(100ng/ml) for 30 minutes whole cell lysate Lane4:BV2 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug)