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V5-tag monoclonal antibody

货号: MB8023

价格：￥

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB8023

Product Name :V5-tag monoclonal antibody

Host :Mouse

Reactivity :Transfected

Applications :WB IP

Application_all :WB: 1:5000~1:20000 IP：1:100~1:1000

Background :Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :V5 mAb detects endogenous levels of V5 protein.

BiowMW :~ 95 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :V5tag

Immunogen :Synthetic peptide, corresponding to V5 tag.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of V5 mAb at 1:5000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate(10ug) Lane3:HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate(20ug) Lane4:HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate(30ug) Lane5:HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate(40ug)

Immunoprecipitation of HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate using V5 mAb (Sepharose Bead Conjugate) #BD0047(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 ) .Lane 1 is 20% input. The western blot was probed using V5 mAb #MB8023.“↑”（supernatant）; “↓”（deposition）