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C9orf72 monoclonal antibody

货号: MB23433

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23433

Product Name :C9orf72 monoclonal antibody

Host :Mouse

Reactivity :Human, Mouse, Monkey, Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :54.3kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ALSFTD; DENND9; FTDALS; DENNL72; FTDALS1

Immunogen :Purified recombinant fragment of human C9orf72 (AA: 110-199) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using C9orf72 mouse mAb against C6 (1), PC-12 (2),COS-7 (3),NIH/3T3 (4) and SK-N-SH (5) cell lysate.

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using C9orf72 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using C9orf72 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using C9orf72 mouse mAb (green) and negative control (red).