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TOP1 monoclonal antibody

货号: MB23412

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23412

Product Name :TOP1 monoclonal antibody

Swiss-Prot :P11387

Host :Mouse

Reactivity :Human, Mouse

Applications :WB,IF,FCM

Application_all :WB:1/500 - 1/2000 IF:1/100 - 1/400 FC:1/200 - 1/400

Background :This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :90.7kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TOPI

Immunogen :Purified recombinant fragment of human TOP1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using TOP1 mouse mAb against HUVEC (1), Jukat (2),MCF-7 (3),PC-12 (4),Hela (5),NIH/3T3 (6) and HCT116 (7) cell lysate.

Immunofluorescence analysis of Hela cells using TOP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)