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P4HB monoclonal antibody

货号: MB23296

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23296

Product Name :P4HB monoclonal antibody

Swiss-Prot :P07237

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :57.1kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DSI,GIT, PDI,PHDB, PDIA1,PO4DB, PO4HB,PROHB,CLCRP1, ERBA2L,P4Hbeta

Immunogen :Purified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using P4HB mouse mAb against Hela (1), PANC-1 (2), MCF-7 (3),THP-1 (4),SW620 (5)and HepG2 (6)cell lysate.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using P4HB mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using P4HB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using P4HB mouse mAb (green) and negative control (red).