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RHOA monoclonal antibody

货号: MB23260

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23260

Product Name :RHOA monoclonal antibody

Swiss-Prot :P61586

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :22kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ARHA; ARH12; RHO12; EDFAOB; RHOH12

Immunogen :Purified recombinant fragment of human RHOA (AA: 1-190) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using RHOA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using RHOA mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using RHOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RHOA mouse mAb (green) and negative control (red).