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USP7 monoclonal antibody

货号: MB23258

价格：￥ 1200

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :MB23258

Product Name :USP7 monoclonal antibody

Swiss-Prot :Q93009

Host :Mouse

Reactivity :Human, Mouse, Rat, Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016]</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :128kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TEF1; HAUSP; HAFOUS

Immunogen :Purified recombinant fragment of human USP7 (AA: 1-208) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using USP7 mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using USP7 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using mouse USP7 mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).