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CD47 monoclonal antibody

货号: MB23067

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB23067

Product Name :CD47 monoclonal antibody

Swiss-Prot :Q08722

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :35.2kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IAP; OA3; MER6

Immunogen :Purified recombinant fragment of human CD47 (AA: 19-141) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CD47 mouse mAb against Jurkat (1), MOLT4 (2), HL-60 (3), Raji (4), Ramos (5), and HEK293 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD47 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using CD47 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using CD47 mouse mAb (green) and negative control (red).