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CD99 monoclonal antibody

货号: MB22980

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22980

Product Name :CD99 monoclonal antibody

Swiss-Prot :P14209

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200-1/1000 IF:1/200 - 1/1000 FC:1/200-1/400

Background :The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :18.8kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MIC2; HBA71; MIC2X; MIC2Y; MSK5X

Immunogen :Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CD99 mouse mAb against THP-1 (1), U937 (2), and MOLT4 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human ewing sarcoma tissues using CD99 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using CD99 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Jurkat cells using CD99 mouse mAb (green) and negative control (red).