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WT1 monoclonal antibody

货号: MB22944

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22944

Product Name :WT1 monoclonal antibody

Swiss-Prot :P19544

Host :Mouse

Reactivity :Human, Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200-1/1000 IF:1/200 - 1/1000 FC:1/200-1/400

Background :This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :49.2kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GUD; AWT1; WAGR; WT33; NPHS4; WIT-2

Immunogen :Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using WT1 mouse mAb against HEK293 (1), COS7 (2), and PC-3 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using WT1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using WT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).