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TOP2A monoclonal antibody

货号: MB22898

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22898

Product Name :TOP2A monoclonal antibody

Swiss-Prot :P11388

Host :Mouse

Reactivity :Human, Rat

Applications :WB,IF,FCM

Application_all :WB:1/500 - 1/2000 IF:1/100 - 1/500 FC:1/200-1/400

Background :This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :174.4kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TOP2; TP2A

Immunogen :Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using TOP2A mouse mAb against PC-12 (1), Hela (2), Jurkat (3), and K562 (4) cell lysate.

Immunofluorescence analysis of Hela cells using TOP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.