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CALR monoclonal antibody

货号: MB22850

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22850

Product Name :CALR monoclonal antibody

Swiss-Prot :P27797

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 FC:1/200 - 1/400

Background :Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :48.1kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RO; CRT; SSA; cC1qR; HEL-S-99n

Immunogen :Purified recombinant fragment of human CALR (AA: 18-417) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CALR mouse mAb against Hela (1), MCF-7 (2), NIH/3T3 (3), HepG2 (4), Jurkat (5), Y-79 (6), and C6 (7) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CALR mouse mAb with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）