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PCK2 monoclonal antibody

货号: MB22728

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22728

Product Name :PCK2 monoclonal antibody

Swiss-Prot :Q16822

Host :Mouse

Reactivity :Human,Rat,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]</p>

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :70.7kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PEPCK; PEPCK2; PEPCK-M

Immunogen :Purified recombinant fragment of human PCK2 (AA: 44-175) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using PCK2 mouse mAb against Jurkat (1), C2C12 (2), Hela (3), HepG2 (4), COS7 (5), and HL-60 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PCK2 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using PCK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using PCK2 mouse mAb (green) and negative control (red).