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IGHM monoclonal antibody

货号: MB22362

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22362

Product Name :IGHM monoclonal antibody

Swiss-Prot :P01871

Host :Mouse

Reactivity :Human

Applications :WB,IHC,FCM

Application_all :WB:1/200 - 1/500 IHC:1/200 - 1/1000 FC:1/200 - 1/400

Background :Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :49.3kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MU; VH; AGM1

Immunogen :Purified recombinant fragment of human IGHM (AA: 310-452) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using IGHM mouse mAb against Raji (1) and Ramos (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using IGHM mouse mAb with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）