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ATG3 monoclonal antibody

货号: MB22334

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22334

Product Name :ATG3 monoclonal antibody

Swiss-Prot :Q9NT62

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :35.9kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :APG3; APG3L; PC3-96; APG3-LIKE

Immunogen :Purified recombinant fragment of human ATG3 (AA: 1-100) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ATG3 mouse mAb against K562 (1), Hela (2), and THP-1 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using ATG3 mouse mAb with DAB staining.

Immunofluorescence analysis of SMMC-7721 cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Jurkat cells using ATG3 mouse mAb (green) and negative control (red).