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HLA-B monoclonal antibody

货号: MB22253

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22253

Product Name :HLA-B monoclonal antibody

Swiss-Prot :P01889

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :40.5kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw;

Immunogen :Purified recombinant fragment of human HLA-B (AA: 241-362) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using HLA-B mouse mAb against Ramos (1) and A431 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using HLA-B mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using HLA-B mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of MCF-7 cells using HLA-B mouse mAb (green) and negative control (red).