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TRIM25 monoclonal antibody

货号: MB22218

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22218

Product Name :TRIM25 monoclonal antibody

Swiss-Prot :Q14258

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/50 - 1/250 FC:1/200 - 1/400

Background :The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :71kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EFP; Z147; RNF147; ZNF147

Immunogen :Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using TRIM25 mouse mAb against MCF-7 (1), Hela (2), K562 (3), A549 (4), and MOLT4 (5) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TRIM25 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using TRIM25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using TRIM25 mouse mAb (green) and negative control (red).