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RAD21 monoclonal antibody

货号: MB22115

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22115

Product Name :RAD21 monoclonal antibody

Swiss-Prot :O60216

Host :Mouse

Reactivity :Human,Rat,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :71.7kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21

Immunogen :Purified recombinant fragment of human RAD21 (AA: 287-403) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using RAD21 mouse mAb against Hela (1), HEK293 (2), K562 (3), C6 (4), *** (5), and COS7 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human brain tissues using RAD21 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using RAD21 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RAD21 mouse mAb (green) and negative control (red).