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DDX3X monoclonal antibody

货号: MB22096

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB22096

Product Name :DDX3X monoclonal antibody

Swiss-Prot :O00571

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey,Rabbit

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500 FC:1/200 - 1/400

Background :The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.<br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :73.2kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DBX; DDX3; HLP2; DDX14; CAP-Rf

Immunogen :Purified recombinant fragment of human DDX3X (AA: 518-661) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using DDX3X mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow cytometric analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).