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GLI1 monoclonal antibody

货号: MB21991

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21991

Product Name :GLI1 monoclonal antibody

Swiss-Prot :P08151

Host :Mouse

Reactivity :Human

Applications :IHC,IF,FCM

Application_all :IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. <br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :118kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GLI

Immunogen :Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using GLI1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)