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ATG5 monoclonal antibody

货号: MB21942

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21942

Product Name :ATG5 monoclonal antibody

Swiss-Prot :Q9H1Y0

Host :Mouse

Reactivity :Human,Mouse

Applications :WB,IF,FCM

Application_all :WB:1/500 - 1/2000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :ATG5 involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :32.4kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ASP; APG5; APG5L; hAPG5; APG5-LIKE

Immunogen :Synthesized peptide of human ATG5 (AA: MTDDKDVLRDVWFGRIc).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ATG5 mouse mAb against Hela (1) and K562 (2) cell lysate.

Immunofluorescence analysis of Hela cells using ATG5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)