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RPS6KA2 monoclonal antibody

货号: MB21866

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21866

Product Name :RPS6KA2 monoclonal antibody

Swiss-Prot :Q15349

Host :Mouse

Reactivity :Human,Mouse

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :83.2kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2

Immunogen :Purified recombinant fragment of human RPS6KA2 (AA: 415-734) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using RPS6KA2 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.

Immunofluorescence analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).