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ACLY monoclonal antibody

货号: MB21684

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21684

Product Name :ACLY monoclonal antibody

Swiss-Prot :P53396

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey,Rabbit

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500 FC:1/200 - 1/400

Background :ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. <br /><br /><br />

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :125kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACL; ATPCL; CLATP

Immunogen :Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.

Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ACLY mouse mAb with DAB staining.

Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).