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NEDD8 monoclonal antibody

货号: MB21567

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21567

Product Name :NEDD8 monoclonal antibody

Swiss-Prot :Q15843

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :9kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NEDD-8

Immunogen :Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using NEDD8 mouse mAb against C6 (1) and Hela (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using NEDD8 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).