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MAPK3 monoclonal antibody

货号: MB21518

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21518

Product Name :MAPK3 monoclonal antibody

Swiss-Prot :P27361

Host :Mouse

Reactivity :Human,Mouse,Rat,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :44kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ERK1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; MGC20180

Immunogen :Purified recombinant fragment of human MAPK3 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MAPK3 mouse mAb against Hela (1), Jurkat (2), RAW264.7 (3), HEK293 (4), K562 (5), NIH/3T3 (6), Cos7 (7) and PC-12 (8) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using MAPK3 mouse mAb with DAB staining.

Immunofluorescence analysis of NIH/3T3 cells using MAPK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Hela cells using MAPK3 mouse mAb (blue) and negative control (red).