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EIF4E monoclonal antibody

货号: MB21453

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21453

Product Name :EIF4E monoclonal antibody

Swiss-Prot :P06730

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :25kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E

Immunogen :Purified recombinant fragment of human EIF4E expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.

Immunofluorescence analysis of GC-7901 cells using EIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).