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CARM1 monoclonal antibody

货号: MB21420

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21420

Product Name :CARM1 monoclonal antibody

Swiss-Prot :Q86X55

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al. 2002 (PubMed 11724789). Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :65kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PRMT4; CARM1

Immunogen :Purified recombinant fragment of human CARM1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CARM1 mouse mAb against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and ovarian cancer tissues (right) using CARM1 mouse mAb with DAB staining.

Immunofluorescence analysis of Hela cells using CRAM1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Lovo cells using CARM1 mouse mAb (green) and negative control (purple).