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HK1 monoclonal antibody

货号: MB21364

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21364

Product Name :HK1 monoclonal antibody

Swiss-Prot :P19367

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :102kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; HK1

Immunogen :Purified recombinant fragment of human HK1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using HK1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3) and NIH/3T3 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human kidney tissues using HK1 mouse mAb with DAB staining.

Immunofluorescence analysis of NIH/3T3 cells using HK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of K562 cells using HK1 mouse mAb (green) and negative control (purple).