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HK2 monoclonal antibody

货号: MB21363

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21363

Product Name :HK2 monoclonal antibody

Swiss-Prot :P52789

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 FC:1/200 - 1/400

Background :The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases .Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :102kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HKII; HXK2; DKFZp686M1669; HK2

Immunogen :Purified recombinant fragment of human HK2 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.