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HAUSP monoclonal antibody

货号: MB21283

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB21283

Product Name :HAUSP monoclonal antibody

Swiss-Prot :Q93009

Host :Mouse

Reactivity :Human

Applications :WB

Application_all :WB:1/500 - 1/2000

Background :USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :128kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TEF1; HAUSP; USP7

Immunogen :Purified recombinant fragment of human HAUSP expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using HAUSP mouse mAb against MCF-7 (1) cell lysate.

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）