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p53 monoclonal antibody

货号: MB21219

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21219

Product Name :p53 monoclonal antibody

Swiss-Prot :P04637

Host :Mouse

Reactivity :Human

Applications :WB,IHC

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000

Background :p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :43.7kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LFS1, TRP53, TP53

Immunogen :Purified recombinant fragment of human p53 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using p53 mouse mAb against HEK293 cell lysate(1).

Immunohistochemical analysis of paraffin-embedded human esophageal cancer (left) and lung cancer (right), showing nuclear localization using p53 mouse mAb with DAB staining.