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MDM4 monoclonal antibody

货号: MB21172

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21172

Product Name :MDM4 monoclonal antibody

Swiss-Prot :O15151

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF

Application_all :IHC:1/200 - 1/1000 IF:1/200 - 1/1000

Background :MDM4, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :55kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HDMX; MDMX; MRP1; MDM4

Immunogen :Purified recombinant fragment of human MDM4 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.

Confocal Immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDM4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.