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CHK2 monoclonal antibody

货号: MB21164

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21164

Product Name :CHK2 monoclonal antibody

Swiss-Prot :O96017

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000

Background :CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :61kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDS1; LFS2; CHEK2

Immunogen :Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CHK2 mouse mAb against cell lysate from various cell types.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 mouse mAb.

Confocal Immunofluorescence analysis of Hela cells using CHK2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.