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Glucose-6-phosphate isomerase monoclonal antibody

货号: MB21150

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21150

Product Name :Glucose-6-phosphate isomerase monoclonal antibody

Swiss-Prot :P06744

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF

Application_all :IHC:1/200 - 1/1000 IF:1/200 - 1/1000

Background :Glucose-6-phosphate isomerase, or phosphoglucose isomerase, also known as GPI. It belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in GPI are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :63kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AMF; NLK; PGI; PHI; GNPI; SA-36; GPI

Immunogen :Purified recombinant fragment of human GPI expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using GPI mouse mAb against HepG2 (1) , SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).

Immunohistochemical analysis of paraffin-embedded human Kidney tissues using GPI mouse mAb.

Confocal Immunofluorescence analysis of L-02 cells using GPI mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.