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Calreticulin monoclonal antibody

货号: MB21142

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21142

Product Name :Calreticulin monoclonal antibody

Swiss-Prot :P27797

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500 FC:1/200 - 1/400

Background :Calreticulin, also known as RO, CRT, SSA, cC1qR, FLJ26680, CALR. Entrez Protein NP_004334. It is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :48kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RO; CRT; SSA; cC1qR; FLJ26680; CALR

Immunogen :Synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using Calreticulin mouse mAb against Hela (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human placenta tissues using Calreticulin mouse mAb.

Confocal Immunofluorescence analysis of 3T3-L1 cells using Calreticulin mouse mAb(green). Blue: DRAQ5 fluorescent DNA dye.

免疫沉淀（IP）