产品类别

联系我们

电话:18013895086

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

AMACR monoclonal antibody

货号: MB21131

价格：￥ 1200

货期：现货（1-2个工作日）

25ug 50ug 100ug 200ug

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB21131

Product Name :AMACR monoclonal antibody

Swiss-Prot :Q9UHK6

Host :Mouse

Reactivity :Human,Mouse

Applications :WB,IHC,IF

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000

Background :AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2b

BiowMW :42kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RACE

Immunogen :Purified recombinant fragment of human AMACR expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using AMACR mouse mAb with DAB staining.

Confocal Immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.