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GFP Tag monoclonal antibody

货号: MB21122

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21122

Product Name :GFP Tag monoclonal antibody

Swiss-Prot :P42212

Host :Mouse

Reactivity :Tag

Applications :WB,IP,IF

Application_all :WB:1/500-1/1000 IHC:1/200 - 1/1000 IF:1/100-1/200 FC:1/200 - 1/400

Background :GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG2a

BiowMW :27kDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Purified recombinant fragment of GFP expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .