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Vimentin monoclonal antibody

货号: MB21098

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21098

Product Name :Vimentin monoclonal antibody

Swiss-Prot :P08670

Host :Mouse

Reactivity :Human,Mouse,Monkey

Applications :WB,IHC,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 FC:1/200 - 1/400

Background :Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :54kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FLJ36605; VIM

Immunogen :Purified recombinant fragment of Vimentin expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using Vimentin mouse mAb against NIH/3T3 cell lysate.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining.