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ER-alpha monoclonal antibody

货号: MB21056

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB21056

Product Name :ER-alpha monoclonal antibody

Swiss-Prot :P03372

Host :Mouse

Reactivity :Human

Applications :WB,IHC

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000

Background :The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.

Product :Purified antibody in PBS with 0.05% sodium azide

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ER; ESR1; Era; ESRA

Immunogen :Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using ER-alpha mouse mAb against human breast carcinoma tissue lysate.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha mouse mAb showing nuclear expression with DAB staining.

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）