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GAPDH monoclonal antibody

货号: MB21049

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21049

Product Name :GAPDH monoclonal antibody

Swiss-Prot :P04406

Host :Mouse

Reactivity :Human,Mouse,Rat,Rabbit,Monkey

Applications :WB,IHC,IF,FCM

Application_all :WB:1/5000- 1/20000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :37kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :G3PD; GAPD; MGC88685;Glyceraldehyde-3-phosphate dehydrogenase

Immunogen :Purified recombinant fragment of human GAPDH expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using GAPDH mouse mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH mouse mAb with DAB staining.

Confocal Immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric analysis of NIH3T3 cells using GAPDH mouse mAb (green) and negative control (red).