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CHK1 monoclonal antibody

货号: MB21046

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB21046

Product Name :CHK1 monoclonal antibody

Swiss-Prot :O14757

Host :Mouse

Reactivity :Human,Mouse

Applications :WB

Application_all :WB:1/500 - 1/2000

Background :CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :56kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CHEK1

Immunogen :Purified recombinant fragment of CHK1 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate.

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）