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MSH2 monoclonal antibody

货号: MB21042

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21042

Product Name :MSH2 monoclonal antibody

Swiss-Prot :P43246

Host :Mouse

Reactivity :Human,Mouse

Applications :WB,IHC,IF

Application_all :WB:1/500 - 1/2000 IHC:1/100 - 1/500 IF:1/50 - 1/500

Background :MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.

Product :Purified antibody in PBS with 0.05% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :105kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FCC1; COCA1; HNPCC; LCFS2

Immunogen :Purified recombinant fragment of human MSH2 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

Confocal Immunofluorescence analysis of Hela cells using MSH2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.