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Nucleophosmin monoclonal antibody

货号: MB21028

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21028

Product Name :Nucleophosmin monoclonal antibody

Swiss-Prot :P06748

Host :Mouse

Reactivity :Human,Monkey

Applications :WB,IHC,IF

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000

Background :Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :33kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :B23; NPM

Immunogen :Purified recombinant fragment of human NPM (2-265) expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM mouse mAb with DAB staining.

Confocal Immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.