Western blot analysis of IL-24 expression in Hela (A), Jurkat (B), K562 (C), A431 (D) whole cell lysates.
Immunohistochemical analysis of IL-24 staining in human melanoma formalin fixed paraffin embedded tissue section.
Flow cytometric analysis of U2OS cells using Anti-IL-24 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
