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ANAPC5 polyclonal antibody

货号: BS61681

价格：￥

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS61681

Product Name :ANAPC5 polyclonal antibody

Swiss-Prot :Q9UJX4

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10 and APC11. The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC1, previously referred to as Tsg24 in mice, is the largest of the APC subunits and is encoded by a gene mapping to 2q12.1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ANAPC5 polyclonal antibody detects endogenous levels of ANAPC5 protein.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Anaphase-promoting complex subunit 5; APC5; Cyclosome subunit 5; ANAPC5; APC5

Immunogen :Synthetic peptide, corresponding Human ANAPC5.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ANAPC5 polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug)