产品类别

联系我们

电话:18013895086

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ERK1/2 (phospho-T202/Y204) polyclonal antibody

货号: BS5016

价格：￥

货期：现货（1-2个工作日）

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS5016

Product Name :ERK1/2 (phospho-T202/Y204) polyclonal antibody

Swiss-Prot :P27361/P28482

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-ERK1/2 (T202/Y204) polyclonal antibody detects endogenous levels of ERK1 protein when phosphorylated at threonine 202 and tyrosine 204, and ERK2 protein when phosphorylated at threonine 185 and tyrosine 187.

BiowMW :~ 42,44 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK1; ERK2; PRKM1; PRKM2

Immunogen :Synthetic phosphopeptide derived from human ERK1/2 around the phosphorylation site of Threonine 202/Tyrosine 204.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-ERK1/2 (T202/Y204) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate treated with UV(4h) Lane2:Raw264.7 whole cell lysate treated with UV(4h) Lane3:PC12 whole cell lysate treated with UV(4h)

Immunohistochemistry (IHC) analyzes of p-ERK1/2 (T202/Y204) pAb in paraffin-embedded human breast carcinoma tissue.

Signal transduction involved in lipoxin A4‑induced protection of tubular epithelial cells against hypoxia/reoxygenation injury

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesHuman

PMID28259922
High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma

JournalOncotarget

ApplicationIHC

IF0

SpeciesHuman

PMID28938613
Establishment of a novel mesenchymal stem cell-based regimen for chronic myeloid leukemia differentiation therapy

JournalCell Death & Disease

ApplicationWB

IF6.304

SpeciesHuman

PMID33627636
Clemastine improves hypomyelination in rats with hypoxic–ischemic brain injury by reducing microglia-derived IL-1β via P38 signaling pathway

JournalJournal of Neuroinflammation

ApplicationWB

IF5.793

SpeciesRat

PMID32061255
Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells

JournalJournal Of Cellular Physiology

ApplicationWB

IF4.522

SpeciesMouse

PMID30628054