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TFEB polyclonal antibody

货号: BS8832

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS8832

Product Name :TFEB polyclonal antibody

Swiss-Prot :P19484

Host :Rabbit

Reactivity :Human, Mouse, Rat, Bovine, Dog, Pig

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200

Background :Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TFEB polyclonal antibody detects endogenous levels of TFEB protein.

BiowMW :65KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BHLHE35; Transcription factor EB; Class E basic helix-loop-helix protein 35; bHLHe35

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TFEB.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of TFEB expression in Myla2059 (A), A549 (B) whole cell lysates.

Immunohistochemical analysis of TFEB staining in human lung cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of TFEB staining in MCF7 cells.

免疫沉淀（IP）